What the accuracy of fields stitching can be achieved when moving the stage in the following cases: using additional markers in each field and without them?
A:
The accuracy of field stitching depends on accuracy of stage movement mostly.
With the help of NanoMaker the sources of inaccuracies like:
disalignment of stage coordinate system and scanning system
distortion of microscope optics (non-liner errors)
the whole system drift
could be measured and accordingly corrected.